The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 24, 2024

Filed:

Jun. 06, 2017
Applicant:

Northwestern University, Evanston, IL (US);

Inventors:

Milan Mrksich, Hinsdale, IL (US);

Justin A. Modica, Chicago, IL (US);

Assignee:

NORTHWESTERN UNIVERSITY, Evanston, IL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 16/46 (2006.01); A61P 19/02 (2006.01); A61P 35/00 (2006.01); A61P 37/00 (2006.01); C07K 16/32 (2006.01); C12N 9/18 (2006.01); A61K 39/00 (2006.01);
U.S. Cl.
CPC ...
C07K 16/46 (2013.01); A61P 19/02 (2018.01); A61P 35/00 (2018.01); A61P 37/00 (2018.01); C07K 16/32 (2013.01); C12N 9/18 (2013.01); C12Y 301/01074 (2013.01); A61K 2039/505 (2013.01); C07K 2317/24 (2013.01); C07K 2317/55 (2013.01); C07K 2317/565 (2013.01); C07K 2317/73 (2013.01); C07K 2317/92 (2013.01); C07K 2317/94 (2013.01); C07K 2319/00 (2013.01); C07K 2319/61 (2013.01); C12Y 201/01037 (2013.01); C12Y 301/21002 (2013.01); C12Y 302/01021 (2013.01); C12Y 304/11018 (2013.01); C12Y 304/24024 (2013.01); C12Y 305/02006 (2013.01);
Abstract

The disclosure provides constructs comprising a first fusion protein, a second fusion protein, and a linker, wherein the first fusion protein and the second fusion protein each include an affinity reagent and a reactive enzyme, and the linker includes a first and second functional groups specific for irreversibly inhibiting the first and second fusion protein reactive enzymes. The disclosure further provides a method including (a) contacting a first fusion protein including an affinity reagent and a reactive enzyme with a linker including a functional group specific for irreversibly inhibiting the first fusion protein reactive enzyme thereby coupling the first fusion protein and the linker, and (b) contacting a second fusion protein including an affinity reagent and a reactive enzyme with the linker, the linker including a functional group specific for irreversibly inhibiting the second fusion protein reactive enzyme thereby coupling the second fusion protein and the linker.


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