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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 12097499 B1

PDFFull Text
Date of Patent:
Sep. 24, 2024

Filed:

Jan. 06, 2023

Determining a bulk concentration of a target in a sample using a digital assay with compartments having nonuniform volumes

Applicant:

Tokitae Llc, Bellevue, WA (US);

Inventors:

Toan Huynh, Seattle, WA (US);

Bernhard Hans Weigl, Seattle, WA (US);

Samantha A. Byrnes, Seattle, WA (US);

Kevin Paul Flood Nichols, Issaquah, WA (US);

Assignee:

Tokitae LLC;

Attorneys:

AEON Law, PLLC

Adam L. K. Philipp

Charlotte E. Holoubek

Primary Examiner:

Jennifer Wecker

Assistant Examiner:

Oyeleye Alexander Alabi

Int. Cl.
CPC ...
B01L 3/00 (2006.01); B01F 23/00 (2022.01); B01F 23/41 (2022.01); B01F 101/23 (2022.01); B23Q 17/24 (2006.01); C12M 1/34 (2006.01); C12Q 1/04 (2006.01); C12Q 1/18 (2006.01); C12Q 1/6816 (2018.01); C12Q 1/686 (2018.01); C23C 2/00 (2006.01); G01N 21/3577 (2014.01); G01N 21/359 (2014.01); G01N 21/39 (2006.01); G01N 21/45 (2006.01); G01N 21/64 (2006.01); G01N 30/12 (2006.01); G01N 30/68 (2006.01); G01N 30/70 (2006.01); G01N 30/72 (2006.01); G01N 30/88 (2006.01); G01N 33/00 (2006.01); G01N 33/18 (2006.01); G01N 33/50 (2006.01); G01N 33/543 (2006.01); G01N 33/68 (2006.01); G01N 35/04 (2006.01); G01N 35/10 (2006.01);
U.S. Cl.
CPC ...
B01L 3/5082 (2013.01); C12Q 1/6816 (2013.01); C23C 2/51 (2022.08); B01L 2200/18 (2013.01); B01L 2300/0663 (2013.01); B01L 2300/0832 (2013.01); B01L 2400/0433 (2013.01); G01N 33/18 (2013.01);
Abstract

An embodiment of a system includes a compartment-generating device, a compartment detector, and electronic computing circuitry. The device is configured to generate compartments of a digital assay, at least one of the compartments having a respective volume that is different from a respective volume of each of at least another one of the compartments. The detector is configured to determine a number of the compartments each having a respective number of a target that is greater than a threshold number of the target. And the electronic circuitry is configured to determine a bulk concentration of the target in a source of the sample in response to the determined number of compartments. Because such a system can be configured to estimate a bulk concentration of a target in a source from a polydisperse digital assay, the system can be portable, and lower-cost and faster, than conventional systems.

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