The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 24, 2024

Filed:

Apr. 26, 2022
Applicants:

Cellseed Inc., Tokyo, JP;

Tokai University Educational System, Tokyo, JP;

Inventors:

Masato Sato, Isehara, JP;

Chikako Sato, Tokyo, JP;

Tomomi Kotoku, Tokyo, JP;

Tomoko Kawake, Tokyo, JP;

Assignees:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/077 (2010.01); A61L 27/36 (2006.01); A61L 27/38 (2006.01); C12N 5/0775 (2010.01);
U.S. Cl.
CPC ...
A61L 27/3612 (2013.01); A61L 27/3817 (2013.01); A61L 27/3834 (2013.01); A61L 27/3852 (2013.01); C12N 5/0655 (2013.01); C12N 5/0668 (2013.01); A61L 2430/06 (2013.01); C12N 2533/30 (2013.01);
Abstract

Provided is a cell sheet suitable for cartilage repair. The present invention provides a cell sheet for cartilage repair, formed from a culture of cells derived from a cartilage tissue, and the cell sheet is negative for immunostaining using an antibody against type II collagen. The present invention also provides a method for producing a cell sheet for cartilage repair, formed from a culture of cells derived from a cartilage tissue, and the method includes culturing cells derived from a cartilage tissue on a surface of a membrane, where a temperature-responsive polymer is immobilized on the surface, to give the cell sheet. The culturing is stopped before the cell sheet becomes positive for immunostaining using an antibody against type II collagen.


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