The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 17, 2024

Filed:

Nov. 01, 2023
Applicant:

10x Genomics, Inc., Pleasanton, CA (US);

Inventors:

Tarjei Sigurd Mikkelsen, Dublin, CA (US);

Eswar Prasad Ramachandran Iyer, Sunnyvale, CA (US);

Andrew Kohlway, Pleasanton, CA (US);

Luigi Jhon Alvarado Martinez, Walnut Creek, CA (US);

Katherine Pfeiffer, Berkeley, CA (US);

Andrew D. Price, Hayward, CA (US);

Assignee:

10X GENOMICS, INC., Pleasanton, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/532 (2006.01); C07K 14/74 (2006.01); C12N 15/10 (2006.01); C12N 15/11 (2006.01); C12N 15/85 (2006.01); C12Q 1/6804 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6818 (2018.01); C12Q 1/6827 (2018.01); C12Q 1/6881 (2018.01); C40B 30/04 (2006.01); C40B 50/06 (2006.01); C40B 70/00 (2006.01); G01N 33/50 (2006.01); G01N 33/53 (2006.01); G01N 33/543 (2006.01); G01N 33/548 (2006.01); G01N 33/569 (2006.01); G01N 33/58 (2006.01);
U.S. Cl.
CPC ...
G01N 33/532 (2013.01); C07K 14/70539 (2013.01); C12N 15/1037 (2013.01); C12N 15/1055 (2013.01); C12N 15/1065 (2013.01); C12N 15/1075 (2013.01); C12N 15/11 (2013.01); C12N 15/85 (2013.01); C12Q 1/6804 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6818 (2013.01); C12Q 1/6827 (2013.01); C12Q 1/6881 (2013.01); G01N 33/5032 (2013.01); G01N 33/505 (2013.01); G01N 33/5304 (2013.01); G01N 33/5306 (2013.01); G01N 33/5308 (2013.01); G01N 33/54306 (2013.01); G01N 33/54366 (2013.01); G01N 33/548 (2013.01); G01N 33/56977 (2013.01); G01N 33/58 (2013.01); C12N 2310/20 (2017.05); C12N 2320/10 (2013.01); C12Q 2537/164 (2013.01); C12Q 2563/179 (2013.01); C12Q 2563/185 (2013.01); C12Q 2565/1015 (2013.01); C40B 30/04 (2013.01); C40B 50/06 (2013.01); C40B 70/00 (2013.01);
Abstract

The present disclosure provides methods of processing or analyzing a sample. A method for processing a sample may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule), barcoding the probe-nucleic acid molecule complex, and performing extension, denaturation, and amplification processes. A method for processing a sample may comprise hybridizing first and second probes to adjacent or non-adjacent target regions of a nucleic acid molecule (e.g., an RNA molecule), linking the first and second probes to provide a probe-linked nucleic acid molecule, and barcoding the probe-linked nucleic acid molecule. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well. One or more processes of the methods described herein may be performed on a cell, such as a permeabilized cell.


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