The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 17, 2024
Filed:
May. 09, 2022
Mbd Co., Ltd., Suwon-si, KR;
Samsung Life Public Welfare Foundation, Seoul, KR;
Research & Business Foundation Sungkyunkwan University, Suwon-si, KR;
Bo Sung Ku, Yongin-si, KR;
Jung Eun Kim, Yongin-si, KR;
Man Ki Chung, Seoul, KR;
Dong Woo Lee, Daejeon, KR;
MBD Co., Ltd., Suwon-si, KR;
SAMSUNG LIFE PUBLIC WELFARE FOUNDATION, Seoul, KR;
RESEARCH & BUSINESS FOUNDATION SUNGKYUNKWAN UNIVERSITY, Suwon-si, KR;
Abstract
The present invention is directed to a method of measuring cell migration by measuring the invasion ratio of cells incubated on a pillar array inserted into a well structure, the method including steps of: preparing a pillar array having a plurality of micropillars and a well structure having a plurality of microwells into which the plurality of micropillars is insertable, respectively; forming cell spheroids by incubating cells in an extracellular matrix attached to the end contact surfaces of the micropillars; allowing the cells contained in the cell spheroids to invade the end contact surfaces; staining and scanning the cell spheroids, the cells contained in the cell spheroids, and the cells that invaded the end contact surfaces; and calculating the invasion ratio of cells by the following equation through a fluorescence image of the scanned cells: wherein Arepresents the total cell area, and Arepresents the spheroid area.