The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 10, 2024
Filed:
Feb. 22, 2022
Weijia Wang, Zhongshan, CN;
Yong Yuan, Zhongshan, CN;
Weijia Wang, Zhongshan, CN;
Qian Dong, Zhongshan, CN;
Kang Chen, Zhongshan, CN;
Xinjie Wang, Zhongshan, CN;
Peng Liu, Zhongshan, CN;
Jinye Xie, Zhongshan, CN;
Yang Li, Zhongshan, CN;
Yiyun Geng, Zhongshan, CN;
Xinyi Liu, Zhongshan, CN;
Juan Wang, Zhongshan, CN;
Yanping Feng, Zhongshan, CN;
Other;
Abstract
The present disclosure discloses a detection kit for, a preparation method and an application thereof, the detection kit includes: amplification primer, crRNA, LbCas12a-RR protein and a single-stranded DNA reporting system, the crRNA is a specific crRNA for a detection segment of the flag gene of; a LbCas12a-RR protein expression sequence is a prokaryotic codon optimized with a Cas12 protein nucleic acid sequence, and amino acid positions of 532 and 595 are mutated into G532R and K595R, respectively. The application of the kit is, to use the detection kit offor nucleic acid detection of; and the kit has high sensitivity, high specificity and fast visualization.