The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 10, 2024

Filed:

Nov. 14, 2022
Applicant:

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

Juan Pablo Maianti, Cambridge, MA (US);

David R. Liu, Cambridge, MA (US);

Luke W. Koblan, Cambridge, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/67 (2006.01); C12N 9/22 (2006.01); C12N 9/78 (2006.01); C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12N 15/67 (2013.01); C12N 9/22 (2013.01); C12N 9/78 (2013.01); C12N 15/102 (2013.01); C07K 2319/81 (2013.01); C12Y 305/04001 (2013.01);
Abstract

Provided herein are systems, compositions, and methods for the incorporation of unnatural amino acids into proteins via nonsense suppression or rare codon suppression. Nonsense codons and rare codons may be introduced into the coding sequence of a protein of interest using a CRISPR/Cas9-based nucleobase editor described herein. The nucleobase editors are able to be programmed by guide nucleotide sequences to edit the target codons in the coding sequence of the protein of interest. Also provided are application enabled by the technology described herein.


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