The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 03, 2024

Filed:

Jul. 14, 2021
Applicant:

Morehouse School of Medicine, Atlanta, GA (US);

Inventors:

Vincent C. Bond, Stone Mountain, GA (US);

Michael Powell, Douglasville, GA (US);

Ming Bo Huang, Atlanta, GA (US);

Syed Ali, Lafayette, LA (US);

Assignee:

MOREHOUSE SCHOOL OF MEDICINE, Atlanta, GA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C07K 14/16 (2006.01); A61K 9/127 (2006.01); A61K 38/16 (2006.01); C07K 14/005 (2006.01); A61K 38/00 (2006.01); A61K 39/00 (2006.01); A61K 39/39 (2006.01); A61K 45/06 (2006.01); C12N 15/00 (2006.01); C12P 21/02 (2006.01); G01N 33/543 (2006.01); G01N 33/566 (2006.01);
U.S. Cl.
CPC ...
C07K 14/163 (2013.01); A61K 9/127 (2013.01); A61K 38/162 (2013.01); C07K 14/005 (2013.01); A61K 38/00 (2013.01); A61K 39/39 (2013.01); A61K 2039/55555 (2013.01); A61K 45/06 (2013.01); C07K 2319/00 (2013.01); C07K 2319/055 (2013.01); C07K 2319/50 (2013.01); C07K 2319/60 (2013.01); C12N 15/00 (2013.01); C12N 2740/16311 (2013.01); C12N 2740/16322 (2013.01); C12P 21/02 (2013.01); G01N 33/543 (2013.01); G01N 33/566 (2013.01);
Abstract

The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest; isolating exosomes from the exosome-producing cell culture; and purifying the protein of interest from the isolated exosomes. The application further discloses compositions that comprise exosomes containing the Nef-fusion protein, as well as methods of using the Nef-fusion protein and exosomes containing the Nef-fusion protein.


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