The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 03, 2024
Filed:
Apr. 07, 2017
Applicant:
The Governing Council of the University of Toronto, Toronto, CA;
Inventors:
Peter W. Zandstra, Toronto, CA;
Shreya Shukla, Toronto, CA;
Assignee:
THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO, Toronto, CA;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
A61K 35/17 (2015.01); C12N 5/074 (2010.01); C12N 5/0783 (2010.01); C12N 5/0789 (2010.01); A61P 7/00 (2006.01); A61P 35/00 (2006.01);
U.S. Cl.
CPC ...
A61K 35/17 (2013.01); C12N 5/0607 (2013.01); C12N 5/0636 (2013.01); C12N 5/0647 (2013.01); C12N 5/0696 (2013.01); A61P 7/00 (2018.01); A61P 35/00 (2018.01); C12N 2500/38 (2013.01); C12N 2500/99 (2013.01); C12N 2501/115 (2013.01); C12N 2501/125 (2013.01); C12N 2501/145 (2013.01); C12N 2501/155 (2013.01); C12N 2501/165 (2013.01); C12N 2501/2307 (2013.01); C12N 2501/25 (2013.01); C12N 2501/42 (2013.01); C12N 2501/58 (2013.01); C12N 2501/998 (2013.01); C12N 2502/1171 (2013.01); C12N 2533/52 (2013.01); C12N 2533/90 (2013.01);
Abstract
The present disclosure provides a method for generating progenitor T cells from stem and/or progenitor cells comprising exposing the stem and/or progenitor cells to Notch ligand Delta-like-4 (DL4) and vascular adhesion molecule 1 (VCAM-1) under conditions suitable to generate progenitor T cells. The method provided is suitable for in vitro and in vivo pro-T cell generation. In vitro, the pro-T cells are generated under serum-free conditions. Cells produced using the method are provided as well as methods of using same.