The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 27, 2024

Filed:

Nov. 02, 2017
Applicants:

University of Iowa Research Foundation, Iowa City, IA (US);

Synderbio, Inc., Coralville, IA (US);

Inventors:

Michael D. Henry, Iowa City, IA (US);

Benjamin Krog, Iowa City, IA (US);

Sarah Vigmostad, Iowa City, IA (US);

Mike Cable, Iowa City, IA (US);

Assignees:

University of Iowa Research Foundation, Iowa City, IA (US);

SynderBio, Inc., Coralville, IA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/569 (2006.01); C12Q 1/6806 (2018.01); G01N 1/28 (2006.01); G01N 1/34 (2006.01); G01N 33/483 (2006.01);
U.S. Cl.
CPC ...
G01N 33/56966 (2013.01); C12Q 1/6806 (2013.01); G01N 1/286 (2013.01); G01N 33/4833 (2013.01); G01N 2001/2866 (2013.01); G01N 1/34 (2013.01);
Abstract

In some embodiments, the present disclosure provides an apparatus and methods for selectively lysing cells from a cell suspension including disassociating a population of cells from a tissue sample, the population of cells including a population of one or more viable cells and a population of one or more dead and dying cells, exposing the population of cells to at least one pulse of fluid shear stress having a force along a conduit wall of from about 500 dyn/cm2 to about 2500 dyn/cm2 to substantially lyse the population of one or more dead or dying cells, leaving the population of one or more viable cells substantially intact, and collecting the population of one or more viable cells.


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