The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 20, 2024
Filed:
Aug. 01, 2022
Victoria Link Limited, Wellington, NZ;
Matthew Joseph Nicholson, Canterbury, NZ;
Sarah Adeline Kessans, Christchurch, NZ;
Emily Jane Parker, Wellington, NZ;
Leyla Yolanda Bustamante Rodriguez, Prebbleton, NZ;
David Barry Scott, Palmerston North, NZ;
Kyle Cornelius Van de Bittner, Wellington, NZ;
Craig John Van Dolleweerd, Prebbleton, NZ;
Massey University, Palmerston, NZ;
Abstract
Nodulisporic acids (NAs) comprise a group of indole diterpenes known for their potent insecticidal activities; however, biosynthesis of NAs by its natural producer,(sp.) is exceptionally difficult to achieve. The identification of genes responsible for NA production could enable biosynthetic pathway optimization to provide access to NAs for commercial applications. Obtaining useful quantities of NAs using published fermentations methods is challenging, making gene knockout studies an undesirable method to confirm gene function. Alternatively, heterologous gene expression ofgenes in a more robust host species likeprovides a way to rapidly identify the function of genes that play a role in NA biosynthesis. In this work, we identified the function of four secondary-metabolic genes necessary for the biosynthesis of nodulisporic acid F (NAF) and reconstituted these genes in the genome ofto enable heterologous production of NAF in this fungus.