The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 13, 2024
Filed:
Aug. 27, 2018
President and Fellows of Harvard College, Cambridge, MA (US);
Ipsen Biopharm Ltd, Wrexham, GB;
The Broad Institute, Inc., Cambridge, MA (US);
Michael S. Packer, Cambridge, MA (US);
Travis R. Blum, Cambridge, MA (US);
David R. Liu, Cambridge, MA (US);
Keith A. Foster, Salisbury, GB;
Matthew Brian Beard, Leighton Buzzard, GB;
President and Fellows of Harvard College, Cambridge, MA (US);
Ipsen Biopharm Ltd, Wrexham, GB;
The Broad Institute, Inc., Cambridge, MA (US);
Abstract
The disclosure provides amino acid sequence variants of Botulinum neurotoxin (BoNT) proteases that cleave (VAMP1, VAMP2, VAMP7, VAMP8, SNAP25, SNAP23, PTEN, etc.) and methods of evolving the same. In some embodiments, proteases described by the disclosure are useful for cleaving proteins found in a cell, that is in an intracellular environment. In some embodiments, proteases described by the disclosure are useful for treating diseases associated with increased or aberrant VAMP7, VAMP8, SNAP23 or PTEN expression or activity, for example, cancer and neurological disorders. Some aspects of this disclosure provide methods for generating BoNT protease variants by continuous directed evolution.