The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 06, 2024

Filed:

Dec. 17, 2019
Applicants:

Bloomage Biotechnology Corporation Limited, Jinan, CN;

Jiangnan University, Wuxi, CN;

Inventors:

Zhen Kang, Wuxi, CN;

Jian Chen, Wuxi, CN;

Litao Hu, Wuxi, CN;

Guocheng Du, Wuxi, CN;

Yang Wang, Wuxi, CN;

Jianghua Li, Wuxi, CN;

Jialian Li, Wuxi, CN;

Tianmeng Zhang, Jinan, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/04 (2006.01); C12N 1/20 (2006.01); C12N 9/10 (2006.01); C12N 9/26 (2006.01); C12N 15/77 (2006.01); C12R 1/15 (2006.01);
U.S. Cl.
CPC ...
C12P 19/04 (2013.01); C12N 1/205 (2021.05); C12N 9/1051 (2013.01); C12N 9/2474 (2013.01); C12N 15/77 (2013.01); C12Y 204/01212 (2013.01); C12Y 302/01035 (2013.01); C12N 2800/101 (2013.01); C12R 2001/15 (2021.05);
Abstract

The invention discloses a recombinantfor efficient synthesis of highly pure hyaluronic acid and oligosaccharides thereof, belonging to the technical field of bioengineering. The recombinantconstructed in the present invention can produce hyaluronic acid with a yield up to 40 g/L, and a crude product purity of 95%. Addition of exogenous hyaluronic acid hydrolase and optimization of the fermentation conditions results in hyaluronic acid oligosaccharides with specific molecular weight, and can further improve the yield of hyaluronic acid to 72 g/L. The invention lays a solid foundation for the efficient synthesis of highly pure hyaluronic acid by microorganisms, and the constructed recombinantis suitable for industrial production and application.


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