The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 06, 2024
Filed:
Feb. 06, 2024
Guangzhou Geneseed Biotech Co., Ltd., Guangzhou, CN;
Ming Liu, Guangzhou, CN;
Qiujie Cai, Guangzhou, CN;
Wanjun Zhang, Guangzhou, CN;
MaoLei Zhang, Guangzhou, CN;
Guangzhou Geneseed Biotech Co., Ltd., Guangzhou, CN;
Abstract
The present invention relates to the technical field of molecular biology, and in particular, to a mutant-type RNase R and a preparation method therefor and application thereof. The mutant-type RNase R provided by the present invention is designated RNase R_ΔM8, an amino acid sequence of which is shown as SEQ ID NO. 5, and a nucleotide sequence encoding the amino acid sequence is shown as SEQ ID NO. 6. Preparation processes of the mutant-type RNase R_ΔM8 provided by the present invention include vector construction, vector transformation, protein induction expression, bacteria collection, protein purification, activity assay, etc. The mutant-type RNase R provided by the present invention improves the expression yield and salt tolerance of RNase R and is beneficial to meeting diverse RNA sample requirements.