The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 30, 2024
Filed:
Apr. 01, 2022
Synaffix B.v., Oss, NL;
Sander Sebastiaan Van Berkel, Lent, NL;
Remon Van Geel, Lithoijen, NL;
Maria Antonia Wijdeven, Lent, NL;
Floris Louis Van Delft, Nijmegen, NL;
Synaffix B.V., Oss, NL;
Abstract
The present invention relates to a process for the enzymatic modification of a glycoprotein. The process comprises the step of contacting a glycoprotein comprising a glycan comprising a terminal GlcNAc-moiety, in the presence of glycosyltransferase that is, or is derived from, a β-(1,4)-N-acetylgalactosaminyltransferase, with a non-natural sugar-derivative nucleotide. The non-natural sugar-derivative nucleotide is according to formula (3): wherein A is selected from the group consisting of —N, —C(O)R, —(CH)C≡C—R, —SH, —SC(O)R, —SC(O)OR, —SC(S)OR, —F, —Cl, —Br —I, —OS(O)R, terminal C-Calkenyl groups, C-Ccycloalkenyl groups, C-Calkadienyl groups, terminal C-Callenyl groups and amino groups. The invention further relates to a glycoprotein obtainable by the process according to the invention, to a bioconjugate that can be obtained by conjugating the glycoprotein with a linker-conjugate, and to β-(1,4)-N-acetylgalactosaminyltransferases that can be used in preparing the glycoprotein according to the invention.