The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 30, 2024
Filed:
Oct. 12, 2023
Jiangnan University, Wuxi, CN;
JIANGNAN UNIVERSITY, Wuxi, CN;
Abstract
Disclosed are a construction method and application of a microorganism capable of realizing high production of lacto-N-tetrose, belonging to the field of microbial genetic engineering. The present disclosure employs a strain which has been constructed in the early stage for efficiently producing a precursor lacto-N-triose II as an original strain to synthesize a key gene of the lacto-N-tetrose via over-expression, thus enabling the strain to have a synthesis capability of producing the lacto-N-tetrose. The present disclosure improves the synthesis of the lacto-N-tetrose by screening a high-efficiency β-1,3-galactosyl transferase gene, and reasonably designing the co-expression of the β-1,3-galactosyl transferase gene and a key UDP-glucose 4 epimerase gene (galE) for strengthening a UDP-galactose pathway on a vector pCDFDuet-1. In a shake flask experiment, the lacto-N-tetrose production capacity ofis 3.04 g/L. The lacto-N-tetrose yield in a 3 L fermentation tank reaches 25.49 g/L. Therefore, the microorganism has an industrial application prospect.