The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 23, 2024
Filed:
Oct. 22, 2016
Applicant:
President and Fellows of Harvard College, Cambridge, MA (US);
Inventors:
David R. Liu, Lexington, MA (US);
Johnny Hao Hu, Cambridge, MA (US);
Assignee:
President and Fellows of Harvard College, Cambridge, MA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/00 (2006.01); C07K 14/32 (2006.01); C12N 9/22 (2006.01); C12N 9/24 (2006.01); C12N 9/78 (2006.01); C12N 15/11 (2006.01); C12N 15/90 (2006.01);
U.S. Cl.
CPC ...
C12N 9/22 (2013.01); C07K 14/32 (2013.01); C12N 9/2497 (2013.01); C12N 9/78 (2013.01); C12N 15/11 (2013.01); C12N 15/907 (2013.01); C12Y 305/04005 (2013.01); C07K 2319/00 (2013.01); C07K 2319/09 (2013.01); C07K 2319/80 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01);
Abstract
Some aspects of this disclosure provide strategies, systems, reagents, methods, and kits that are useful for engineering Cas9 and Cas9 variants that have increased activity on target sequences that do not contain the canonical PAM sequence. In some embodiments, fusion proteins comprising such Cas9 variants and nucleic acid editing domains, e.g., deaminase domains, are provided.