The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 16, 2024
Filed:
Jul. 13, 2019
Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, CN;
Bin Yao, Beijing, CN;
Xiaoyun Su, Beijing, CN;
Fei Gao, Beijing, CN;
Huiying Luo, Beijing, CN;
Huoqing Huang, Beijing, CN;
Yingguo Bai, Beijing, CN;
Yuan Wang, Beijing, CN;
Tao Tu, Beijing, CN;
Yaru Wang, Beijing, CN;
Kun Meng, Beijing, CN;
Abstract
The present invention relates to the field of genetic engineering, particularly to a recombinant expression vector for rapidly screening the high expression strains and a method for rapidly screening high expression strains. In the invention, an exogenous red fluorescent protein andcell surface protein localization signal are fused and expressed, and the fusion gene (DsRed-AfMP1) is integrated into the genome of, so as to construct a strain displaying red fuorescent protein on the surface of. By sortingstrains with red fluorescent protein on the surface by flow cytometry, genes beneficial to the improvement of cellulase activity can be quickly isolated.