The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 16, 2024

Filed:

Sep. 15, 2021
Applicant:

Archer Daniels Midland Company, Decatur, IL (US);

Inventors:

Benjamin Mason, Decatur, IL (US);

Jason Helvey, Decatur, IL (US);

Assignee:

ARCHER-DANIELS-MIDLAND COMPANY, Decatur, IL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 13/08 (2006.01); C07K 14/245 (2006.01); C12N 1/20 (2006.01); C12N 9/00 (2006.01); C12N 9/80 (2006.01); C12N 9/88 (2006.01); C12R 1/19 (2006.01);
U.S. Cl.
CPC ...
C12P 13/08 (2013.01); C07K 14/245 (2013.01); C12N 1/205 (2021.05); C12N 9/80 (2013.01); C12N 9/88 (2013.01); C12N 9/93 (2013.01); C12R 2001/19 (2021.05); C12Y 305/01003 (2013.01); C12Y 403/01019 (2013.01); C12Y 604/01001 (2013.01);
Abstract

Improved production of threonine fromby fermentation is accomplished by attenuation but not elimination of the expression of either or both of the yajV gene encoding omega-amidase (a.k.a. 2-oxoglutaramate amidase) and the ilvA gene encoding threonine dehydratase (a.k.a threonine deaminase). In cases where there is attenuated expression of the ilvA gene, there is no need to express an exogenous cimA gene. In examples of both cases, attenuation is accomplished by engineering these genes to contain a weaker ribosome site. Further improvements in threonine production are made by expression of a heterologous pyruvate carboxylase gene exemplified by expression of thepyc gene under control of anpromoter, to provide expression of pyruvate carboxylase that is not naturally expressed in


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