The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 16, 2024
Filed:
Jun. 03, 2020
Nantong Licheng Biological Engineering Co. Ltd, Jiangsu, CN;
Hongzhi Xia, Jiangsu, CN;
Long Liu, Jiangsu, CN;
Qinqing Gu, Jiangsu, CN;
Jiangbo Li, Jiangsu, CN;
Shixiu Cui, Jiangsu, CN;
Xueqin Lv, Jiangsu, CN;
Jianghua Li, Jiangsu, CN;
Guocheng Du, Jiangsu, CN;
Jian Chen, Jiangsu, CN;
NANTONG LICHENG BIOLOGICAL ENGINEERING CO. LTD, Jiangsu, CN;
Abstract
The present disclosure provides a recombinantfor increasing the yield of menaquinone 7 (MK-7) and application thereof, and belongs to the field of genetic engineering. In the present disclosure, 14 recombinant strains BS1-BS14 are constructed through the modification of genes related to the biosynthetic pathway of MK-7 on a chromosome of, wherein BS6-BS14 significantly increase the yield of the MK-7, reaching up to 33.5 mg/L, which is 3.53 times the yield of the original strain of wild-type168. The present disclosure further provides a method for modifying the MK-7 biosynthetic pathway in microorganisms to increase the yield of the MK-7, providing a theoretical basis for constructing a high-yielding strain of the MK-7.