The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 16, 2024

Filed:

Aug. 15, 2023
Applicant:

Roche Sequencing Solutions, Inc., Pleasanton, CA (US);

Inventors:

Cynthia Cech, Mountain View, CA (US);

Tim Craig, Mountain View, CA (US);

Christos Tzitzilonis, Mountain View, CA (US);

Alexander Yang, Mountain View, CA (US);

Liv Jensen, Mountain View, CA (US);

Charlotte Yang, Mountain View, CA (US);

Corissa Harris, Mountain View, CA (US);

Matthew Dipietro, Gilroy, CA (US);

Dhruti Dalal, Sunnyvale, CA (US);

Assignee:

Roche Sequencing Solutions, Inc., Pleasanton, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); A61K 39/02 (2006.01); C07K 14/245 (2006.01); C12N 9/12 (2006.01); C12Q 1/6874 (2018.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
C07K 14/245 (2013.01); C12N 9/1252 (2013.01); C12Q 1/68 (2013.01); C12Q 1/6874 (2013.01); C12Y 207/07007 (2013.01); G01N 33/6803 (2013.01); C07K 2319/00 (2013.01);
Abstract

The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.


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