Microchip and manufacturing method therefor, and multi-channel fluorescence detection device

Abstract

Efficient simultaneous laser-irradiation-fluorescence detection is performed for a plurality of channels of a microchip and simple and highly sensitive parallel analysis of a plurality of samples is enabled. In a microchipmade of an optically transparent solid material mwith a refractive index n, a plurality of channelsfilled with a material mwith a refractive index nand a plurality of channelsfilled with a material mwith a refractive index nare alternately arranged in parallel on the same plane. Here, m, mand mare selected such that a relation of n<n<nis satisfied. The material mis an optically transparent liquid suitable for targeted analysis. When a laser beamis focused and irradiated perpendicularly to the individual channelsandalong the same plane, refraction of the laser beamby the channelsand refraction of the laser beamby the channelsare counterbalanced. For this reason, the laser beampropagates linearly on the same plane and the individual channelsandcan be simultaneously and efficiently irradiated with the laser beam. Fluorescence emitted from the individual channelsandis detected independently and simultaneously using a collimating lens, a filter and a diffraction grating, an imaging lens, a two-dimensional sensor, and a data analysis system, from a direction perpendicular to the same plane.