The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 02, 2024
Filed:
Jan. 10, 2019
Applicant:
Hoffmann-la Roche Inc., Little Falls, NJ (US);
Inventors:
Simone Dethloff, Bichl, DE;
Erhard Kopetzki, Penzberg, DE;
Dominique Ostler, Aidling, DE;
Stefan Seeber, Sindelsdorf, DE;
Georg Tiefenthaler, Sindelsdorf, DE;
Assignee:
HOFFMANN-LA ROCHE INC., Little Falls, NJ (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/85 (2006.01); C07K 16/00 (2006.01); C12N 5/0781 (2010.01); C12N 15/10 (2006.01); C12P 21/02 (2006.01);
U.S. Cl.
CPC ...
C07K 16/00 (2013.01); C12N 5/0635 (2013.01); C12N 15/1086 (2013.01); C12N 15/85 (2013.01); C12P 21/02 (2013.01); C07K 2317/14 (2013.01); C07K 2317/56 (2013.01); C12N 2510/02 (2013.01);
Abstract
In the method as reported herein the isolation of nucleic acid segments encoding antibody variable domains and the insertion of the isolated nucleic acid segments in eukaryotic expression plasmids is performed without the intermediate isolation and analysis of clonal intermediate plasmids. Thus, in the method as reported herein the intermediate cloning, isolation and analysis of intermediate plasmids is not required, e.g. by analysis of isolated transformedcells.