The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 25, 2024

Filed:

Jan. 29, 2020
Applicant:

Arizona Board of Regents on Behalf of the University of Arizona, Tucson, AZ (US);

Inventors:

Leilei Peng, Tucson, AZ (US);

Jun Ding, Palo Alto, CA (US);

Dongli Xu, Palo Alto, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G06T 7/73 (2017.01); G01N 21/64 (2006.01); G02B 21/00 (2006.01); G02B 21/36 (2006.01); G06T 7/11 (2017.01); G06T 7/62 (2017.01); H04N 23/67 (2023.01);
U.S. Cl.
CPC ...
G06T 7/73 (2017.01); G01N 21/6402 (2013.01); G01N 21/6428 (2013.01); G01N 21/6456 (2013.01); G02B 21/0076 (2013.01); G02B 21/365 (2013.01); G06T 7/11 (2017.01); G06T 7/62 (2017.01); H04N 23/671 (2023.01); G01N 2021/6441 (2013.01); G01N 2201/06113 (2013.01); G06T 2207/10056 (2013.01);
Abstract

A microscopic technique for generating high-clarity, large volume 3D images of fluorescent tissue structure at subcellular resolution and capture transient activities. The technique includes capturing two orthogonal 2D projection of the sample volume by performing a projection scan with an excitation laser sweeping through the volume at up to 100 vps, tracking the scan depth using an electrically tuned lens to keep the emission image in focus and generate an xy plane volume projection image at the camera; and placing a PMT behind the excitation lens to collect emission passed through the excitation lens, wherein signals from the PMT form a focus scan projection at the yz plane; and then merging the xy and yz projections.


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