The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 25, 2024

Filed:

Sep. 04, 2020
Applicants:

CZ Biohub Sf, Llc, San Francisco, CA (US);

The Regents of the University of California, Oakland, CA (US);

Inventors:

Jason Peters, Madison, WI (US);

Carol Gross, Berkeley, CA (US);

Oren Rosenberg, San Francisco, CA (US);

Neha Prasad, San Francisco, CA (US);

Assignees:

CZ Biohub SF, LLC, San Francisco, CA (US);

The Regents of the University of California, Oakland, CA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/113 (2010.01); C12N 9/22 (2006.01); C12N 15/70 (2006.01);
U.S. Cl.
CPC ...
C12N 15/113 (2013.01); C12N 9/22 (2013.01); C12N 15/70 (2013.01); C12N 2310/20 (2017.05);
Abstract

Described in this disclosure are CRISPRi systems and methods, along with the related compositions and kits, that combine modularity, stable genomic integration, and ease of transfer to diverse bacteria by conjugation. CRISPRi compositions, methods, systems and kits described herein allow for genetic dissection of bacteria, facilitating analyses of microbiome function, antibiotic resistances and sensitivities, as well as comprehensive screening for host-microbe interactions. Embodiments of the invention comprise compositions, methods, systems, and kits for CRISPRi-based repression of gene expression in bacteria.


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