The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 18, 2024

Filed:

Jul. 17, 2019
Applicant:

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

George M. Church, Brookline, MA (US);

Francois Vigneault, Medford, MA (US);

Uri Laserson, Boston, MA (US);

Ido Bachelet, Brookline, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C07K 16/00 (2006.01); C07K 16/06 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6881 (2018.01); C12Q 1/6883 (2018.01); C40B 10/00 (2006.01); C40B 30/04 (2006.01); C40B 40/08 (2006.01); G16B 20/00 (2019.01); G16B 30/00 (2019.01); C12Q 1/6874 (2018.01);
U.S. Cl.
CPC ...
C40B 10/00 (2013.01); C07K 16/00 (2013.01); C12N 15/10 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6881 (2013.01); C40B 30/04 (2013.01); C40B 40/08 (2013.01); G16B 20/00 (2019.02); G16B 30/00 (2019.02); C07K 16/065 (2013.01); C12Q 1/6874 (2013.01); C12Q 1/6883 (2013.01); C12Q 2535/00 (2013.01); C12Q 2535/122 (2013.01); C12Q 2600/112 (2013.01); C12Q 2600/158 (2013.01);
Abstract

This disclosure provides methods for monitoring an immune response. Methods comprise linking a polynucleotide sequence encoding a heavy chain variable region and a polynucleotide sequence encoding a light chain variable region from a single lymphocyte from a biological sample obtained before an immune response and linking a polynucleotide sequence encoding a heavy chain variable region and a polynucleotide sequence encoding a light chain variable region from a single lymphocyte from a biological sample obtained during or after an immune response. Methods further comprise performing high-throughput sequencing of the linked (paired) sequences from before the immune response and from during or after the immune response, and comparing the resulting sequence reads.


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