The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 18, 2024

Filed:

Nov. 30, 2018
Applicant:

Public University Corporation Yokohama City University, Kanagawa, JP;

Inventors:

Hideki Taniguchi, Yokohama, JP;

Takanori Takebe, Yokohama, JP;

Keisuke Sekine, Yokohama, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/071 (2010.01); A61L 27/36 (2006.01); C12N 5/074 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0697 (2013.01); A61L 27/3625 (2013.01); A61L 27/3641 (2013.01); C12N 5/0607 (2013.01); A61L 2430/28 (2013.01); C12N 2502/1358 (2013.01); C12N 2502/28 (2013.01); C12N 2506/03 (2013.01);
Abstract

The present invention solves the following problems [1] to [3] found in conventional methods of preparing a three dimensional structure (organ primordium) by coculturing functional cells with umbilical cord-derived vascular endothelial cells and bone marrow-derived mesenchymal cells: [1] the quality of resultant organ primordia varies greatly depending on donors; [2] the growth capacities of cell sources are limited; and [3] it is difficult to secure immunocompatibility because cells are derived from different sources. An organ bud prepared from vascular cells, mesenchymal cells and tissue or organ cells, wherein each of the vascular cell, the mesenchymal cell and the tissue or organ cell has been induced from pluripotent stem cells. A method of preparing an organ bud, comprising culturing vascular cells, mesenchymal cells and tissue or organ cells in vitro, wherein each of the vascular cell, the mesenchymal cell and the tissue or organ cell has been induced from pluripotent stem cells.


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