The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 18, 2024

Filed:

Oct. 10, 2017
Applicant:

Pioneer Hi-bred International, Inc., Johnston, IA (US);

Inventors:

Huirong Gao, Johnston, IA (US);

Bailin Li, Johnston, IA (US);

Robert B Meeley, Des Moines, IA (US);

Leandro Daniel Perugini, Urbandale, IA (US);

Girma M Tabor, Johnston, IA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/82 (2006.01);
U.S. Cl.
CPC ...
C12N 15/82 (2013.01); C12N 15/8213 (2013.01); C12N 15/8282 (2013.01);
Abstract

Compositions and methods for obtaining plant cells with modified Ht1 nucleotide sequences, modified NLB18 sequences, or both, are provided herein. The methods involve introducing double-strand breaks into the maize genome in an endogenous Ht1 encoding sequence, an endogenous NLB18 encoding sequence, or both, to modify the genomic sequence in order to enhance northern leaf blight resistance of a plant produced from the plant cell. Further provided are methods that introduce resistant alleles of Ht1 and/or NLB18 into specific sites in the genome. Plants produced by the plant cells, and seeds produced from the plants are also included. Guide polynucleotides are also provided for the use of the CRISPR-Cas system in inducing double strand breaks.


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