The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 28, 2024
Filed:
Jan. 04, 2019
Massachusetts Institute of Technology, Cambridge, MA (US);
The Brigham and Women's Hospital, Inc., Boston, MA (US);
Benjamin E. Mead, Cambridge, MA (US);
Jose Ordovas-Montanes, Cambridge, MA (US);
Alexander K. Shalek, Cambridge, MA (US);
Jeffrey Karp, Boston, MA (US);
Robert Langer, Cambridge, MA (US);
Massachusetts Institute of Technology, Cambridge, MA (US);
The Brigham and Women's Hospital, Inc., Boston, MA (US);
Abstract
Disclosed here is a generally applicable framework that utilizes massively-parallel single-cell RNA-seq to compare cell types/states found in vivo to those of in vitro models. Furthermore, Applicants leverage identified discrepancies to improve model fidelity. Applicants uncover fundamental gene expression differences in lineage-defining genes between in vivo systems and in vitro systems. Using this information, molecular interventions are identified for rationally improving the physiological fidelity of the in vitro system. Applicants demonstrated functional (antimicrobial activity, niche support) improvements in Paneth cell physiology using the methods.