The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 28, 2024

Filed:

Aug. 06, 2019
Applicant:

Wake Forest University Health Sciences, Winston-Salem, NC (US);

Inventors:

Aleksander Skardal, Clemmons, NC (US);

Casey Cuvan Clark, Winston-Salem, NC (US);

Assignee:

Wake Forest University Health Sciences, Winston-Salem, NC (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/071 (2010.01); B33Y 10/00 (2015.01); B33Y 70/00 (2020.01); B33Y 80/00 (2015.01); C12M 3/00 (2006.01); C12N 5/00 (2006.01); C12N 13/00 (2006.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0062 (2013.01); B33Y 10/00 (2014.12); B33Y 70/00 (2014.12); B33Y 80/00 (2014.12); C12M 21/08 (2013.01); C12N 5/0697 (2013.01); C12N 13/00 (2013.01); G01N 33/5088 (2013.01); C12N 2513/00 (2013.01);
Abstract

Provided are methods of preparing organoids and compositions for use in such methods. In some embodiments, a method of depositing a plurality of the cells into a reservoir are provided, the method comprising depositing a gelatin composition into a reservoir; adding a composition comprising a plurality of cells into the gelatin composition, wherein the composition is added into the gelatin composition at a position below the surface of the gelatin composition and at least a portion of the composition and/or plurality of cells are suspended in the gelatin composition; curing at least a portion of the composition comprising the plurality of cells in the gelatin composition; and removing at least a portion of the gelatin composition in the reservoir, thereby depositing the plurality of cells in the reservoir.


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