The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 21, 2024

Filed:

Jan. 11, 2021
Applicant:

Life Technologies Corporation, Carlsbad, CA (US);

Inventors:

Jonathan Schultz, Guilford, CT (US);

Todd Roswech, Westbrook, CT (US);

Jon A. Hoshizaki, Cupertino, CA (US);

Albert L. Carrillo, South San Francisco, CA (US);

James A Ball, Ledyard, CT (US);

Assignee:

Life Technologi s Corporation, Carlsbad, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6869 (2018.01); B01F 1/00 (2006.01); B01F 11/00 (2006.01); B01F 21/20 (2022.01); B01F 25/451 (2022.01); B01F 31/31 (2022.01); B01F 31/65 (2022.01); B01L 3/00 (2006.01); C12Q 1/6806 (2018.01); G01N 27/414 (2006.01); G01N 35/10 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6869 (2013.01); B01F 21/22 (2022.01); B01F 25/451 (2022.01); B01F 31/311 (2022.01); B01F 31/65 (2022.01); B01L 3/502 (2013.01); B01L 3/527 (2013.01); C12Q 1/6806 (2013.01); G01N 27/4145 (2013.01); G01N 35/1002 (2013.01); G01N 35/1095 (2013.01); B01L 3/505 (2013.01); B01L 3/563 (2013.01); B01L 2200/04 (2013.01); B01L 2200/16 (2013.01); B01L 2300/022 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/123 (2013.01); B01L 2400/0481 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0655 (2013.01); B01L 2400/0683 (2013.01); Y10T 436/11 (2015.01); Y10T 436/143333 (2015.01);
Abstract

A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.


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