The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 14, 2024
Filed:
Oct. 25, 2018
Cellpharma Kft., Budapest, HU;
Természettudományi Kutatóközpont, Budapest, HU;
Edit Szabó, Budapest, HU;
Dóra Kovács-Türk, Budapest, HU;
Ágnes Telbisz, Budapest, HU;
Nóra Kucsma, Budapest, HU;
Tamás Horváth, Budapest, HU;
Gergely Szakács, Budapest, HU;
László Homolya, Telki, HU;
Balázs Sarkadi, Budapest, HU;
Várady György, Budapest, HU;
CELLPHARMA KFT., Budapest, HU;
TERMÉSZETTUDOMÁNYI KUTATÓKÖZPONT, Budapest, HU;
Abstract
ABC multidrug transporters are key players in cancer multidrug resistance and in general xenobiotic elimination, thus their functional assays provide important tools for research and diagnostic applications. It has been found that in cells expressing functional ABCG2, ABCB1, or ABCC1 transporters, cellular PG fluorescence is strongly reduced. The invention relates to methods and uses of fluorescein derivative ester compounds of formula Ia which are analogs of PG for assessing ABC transporter activity of ABC multidrug transporters. The present accumulation assay is a novel tool for the parallel determination of the function of the multidrug transporters, in particular ABCG2, ABCB1, and ABCC1. The assay is applicable for diagnostic purposes and also allows the selection, separation and culturing of selected cell populations expressing such transporters.