The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 14, 2024

Filed:

Feb. 27, 2023
Applicant:

Angle Europe Limited, Guildford, GB;

Inventors:

Alexandre Izmailov, Etobicoke, CA;

Brian Pak, Richmond Hill, CA;

Tomoyuki Sen, Toronto, CA;

Paul Timothy Smith, Acton, CA;

Assignee:

ANGLE EUROPE LIMITED, Guildford, GB;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 1/30 (2006.01); B01L 3/00 (2006.01);
U.S. Cl.
CPC ...
G01N 1/30 (2013.01); B01L 3/502753 (2013.01); B01L 3/502761 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/0822 (2013.01); B01L 2300/0832 (2013.01); B01L 2400/088 (2013.01);
Abstract

The present disclosure provides a method of cell sedimentation and retention of target cells, for example circulating tumour cells, CTC, from a fluid sample onto a solid support. The method comprises placing a fluid medium comprising the target cells in a fluid chamber, the fluid chamber having an open end sealed against a surface of the solid support. The method further comprises subjecting the fluid medium to centrifugation within the fluid chamber to induce sedimentation of the target cells and promote cell adhesion to the surface of the solid support. The method further comprises, post-centrifugation, positioning a fluid absorbing element in the fluid chamber to remove fluid from the fluid chamber. The method further comprises controlling a flow rate of the fluid being absorbed by the fluid absorbing element such that the sedimented cells are not detached from the surface of the solid support.


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