The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 14, 2024

Filed:

Sep. 01, 2022
Applicant:

International N&h Denmark Aps, Kongens Lyngby, DK;

Inventors:

Luis Fernando Romero Millan, Wilshire, GB;

Susan Arent Lund, Brabrand, DK;

Brian Soegaard Laursen, Kalundborg, DK;

Elijah Gituanjah Kiarie, Waukesha, WI (US);

Zhenghong Zhang, Shanghai, CN;

Rosalyn Lau, Shanghai, CN;

Zheyong Yu, Shanghai, CN;

Floor Klaaske Kooy, Leiden, NL;

Jan Hendrik Van Tuijl, Zoetermeer, NL;

Bart Christiaan Koops, Pijnacker, NL;

Mads Brogger Pedersen, Frederiksberg, DK;

Soren Dalsgaard, Silkeborg, DK;

Assignee:

INTERNATIONAL N&H DENMARK APS, Kongens Lyngby, DK;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/24 (2006.01); A23K 10/10 (2016.01); A23K 10/14 (2016.01); A23K 10/38 (2016.01); A23K 50/30 (2016.01); A23K 50/75 (2016.01); C12C 5/00 (2006.01); C12P 5/00 (2006.01); C12P 7/10 (2006.01); C12P 19/14 (2006.01); C13K 1/02 (2006.01); C13K 1/06 (2006.01);
U.S. Cl.
CPC ...
C12N 9/2482 (2013.01); A23K 10/10 (2016.05); A23K 10/14 (2016.05); A23K 10/38 (2016.05); A23K 50/30 (2016.05); A23K 50/75 (2016.05); C12C 5/004 (2013.01); C12P 5/007 (2013.01); C12P 7/10 (2013.01); C12P 19/14 (2013.01); C12Y 302/01008 (2013.01); C13K 1/02 (2013.01); C13K 1/06 (2013.01); Y02E 50/10 (2013.01); Y02P 60/87 (2015.11);
Abstract

The present invention relates to a method for solubilising arabinoxylan-containing material (particularly insoluble arabinoxylan-containing material), comprising admixing a xylan-containing material with a xylanase comprising a polypeptide sequence shown herein as SEQ ID No. 3, SEQ ID No. 2, SEQ ID No. 1, SEQ ID No. 9, SEQ ID No. 10. SEQ ID No. 11 or SEQ ID No. 15, or a variant, homologue, fragment or derivative thereof having at least 75% identity with SEQ ID No. 3 or SEQ ID No. 2 or SEQ ID No. 1 or SEQ ID No. 9 or SEQ ID No. 10 or SEQ ID No. 11 or SEQ ID No. 15; or a polypeptide sequence which comprises SEQ ID No. 3, SEQ ID No. 2, SEQ ID No. 1, SEQ ID No. 9, SEQ ID No. 10. SEQ ID No. 11 or SEQ ID No. 15 with a conservative substitution of at least one of the amino acids; or a xylanase which is encoded by a nucleotide sequence shown herein as SEQ ID No. 6, SEQ ID No. 5, SEQ ID No. 4, SEQ ID No. 12. SEQ ID No. 13. SEQ ID No. 14. SEQ ID No. 16. SEQ ID No. 17 or SEQ ID No. 18, or a nucleotide sequence which can hybridize to SEQ ID No. 6, SEQ ID No. 5, SEQ ID No. 4, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14. SEQ ID No. 16. SEQ ID No. 17 or SEQ ID No. 18 under high stringency conditions, or a nucleotide sequence which has at least 75% identity with SEQ ID No. 6, SEQ ID No. 5, SEQ ID No. 4, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, SEQ ID No. 16. SEQ ID No. 17 or SEQ ID No. 18, or a nucleotide sequence which differs from SEQ ID No. 6 or SEQ ID No. 5 or SEQ ID No. 4 or SEQ ID No. 12 or SEQ ID No. 13 or SEQ ID No. 14 or SEQ ID No. 16 or SEQ ID No. 17 or SEQ ID No. 18 due to the degeneracy of the genetic code, or a xylanase obtainable (or obtained) from. The present invention also relates to a novel xylanase comprising (or consisting of) a polypeptide sequence shown herein as SEQ ID No. 3, SEQ ID No. 2 or SEQ ID No. 1, or a variant, homologue, fragment or derivative thereof having at least 99% identity with SEQ ID No. 3 or SEQ ID No. 2 or SEQ ID No. 1; or a xylanase which is encoded by a nucleotide sequence shown herein as SEQ ID No. 6, SEQ ID No. 5 or SEQ ID No. 4, or a nucleotide sequence which can hybridize to SEQ ID No. 4 or SEQ ID No. 5 under high stringency conditions, or a nucleotide sequence which has at least 97.7% identity (preferably 98% identity) with SEQ ID No. 6, SEQ ID No. 5 or SEQ ID No. 4. The present invention yet further relates to methods relating to feedstuffs, malting and brewing, processing of grain-based materials such as during the production of bioethanol or biochemical (e.g. bio-based isopropanol), or wheat gluten-starch separation processes and the like.


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