The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 30, 2024

Filed:

Jun. 04, 2020
Applicant:

Berkeley Lights, Inc., Emeryville, CA (US);

Inventors:

Kevin T. Chapman, Emeryville, CA (US);

Mark P. White, Orinda, CA (US);

Xiaohua Wang, Pomona, NY (US);

Minha Park, Brisbane, CA (US);

Guido K. Stadler, San Francisco, CA (US);

Randall D. Lowe, Jr., Emeryville, CA (US);

Xiao Guan Radstrom, San Rafael, CA (US);

Jason M. McEwen, El Cerrito, CA (US);

Gang F. Wang, Mountain View, CA (US);

George L. Fox, Albany, CA (US);

Peggy A. Radel, Berkeley, CA (US);

Assignee:

Bruker Cellular Analysis, Inc., Emeryville, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); C07K 16/00 (2006.01); C07K 16/28 (2006.01); C07K 16/30 (2006.01); C12Q 1/6886 (2018.01); G01N 33/50 (2006.01); G01N 33/543 (2006.01); G01N 33/569 (2006.01); G01N 33/574 (2006.01);
U.S. Cl.
CPC ...
G01N 33/574 (2013.01); B01L 3/502761 (2013.01); C07K 16/00 (2013.01); C07K 16/2887 (2013.01); C07K 16/30 (2013.01); C12Q 1/6886 (2013.01); G01N 33/5047 (2013.01); G01N 33/505 (2013.01); G01N 33/5052 (2013.01); G01N 33/54366 (2013.01); G01N 33/56972 (2013.01); B01L 2200/0647 (2013.01); G01N 2800/7028 (2013.01);
Abstract

A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.


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