The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 30, 2024

Filed:

Jan. 30, 2020
Applicant:

The General Hospital Corporation, Boston, MA (US);

Inventors:

Eduardo Reategui, Revere, MA (US);

Shannon L. Stott, Stoneham, MA (US);

Mehmet Toner, Charlestown, MA (US);

Eugene J. Lim, Cambridge, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/543 (2006.01); G01N 33/544 (2006.01); G01N 33/569 (2006.01); G01N 33/574 (2006.01);
U.S. Cl.
CPC ...
G01N 33/54393 (2013.01); G01N 33/54366 (2013.01); G01N 33/544 (2013.01); G01N 33/56966 (2013.01); G01N 33/57492 (2013.01); G01N 2333/71 (2013.01); G01N 2400/00 (2013.01);
Abstract

Systems, methods, and devices for selective capture and release of target particles, e.g., living cells, from liquid samples, e.g., blood, are provided. The particle capture systems include a substrate; a first layer of gelatin bound to the substrate by physical adsorption, wherein the gelatin is functionalized with a plurality of first members of a binding pair; a second layer of gelatin wherein the gelatin is functionalized with a plurality of the first members of the binding pair and the second layer is bound to the first layer via a plurality of second members of the binding pair that are associated with the first members of the binding pair on both the first and the second layers; and a plurality of nanostructures bound to the second members of the binding pair and to one or more particle-binding moieties that selectively bind to the target particles.


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