The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 30, 2024

Filed:

Nov. 16, 2020
Applicant:

Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Hubei, CN;

Inventors:

Peiwu Li, Hubei, CN;

Hui Li, Hubei, CN;

Jun Jiang, Hubei, CN;

Wen Zhang, Hubei, CN;

Qi Zhang, Hubei, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/577 (2006.01); G01N 33/533 (2006.01);
U.S. Cl.
CPC ...
G01N 33/533 (2013.01); G01N 33/577 (2013.01);
Abstract

A time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, aflatoxin B1 and sterigmatocystin includes an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing europium-labeled anti-4,15-diacetoxyscirpenol, anti-aflatoxin B1 and anti-sterigmatocystin monoclonal antibodies, where a water absorption pad, a detection pad and a sample pad are sequentially disposed on one side of the immunochromatography time-resolved fluorescence test strip from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a quality control line and detection lines are transversely arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with a rabbit antimouse polyclonal antibody, and the three detection lines each are coated with a toxin-protein conjugate. The three mycotoxins including 4,15-diacetoxyscirpenol, aflatoxin B1 and sterigmatocystin can be rapidly and synchronously detected.


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