The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 23, 2024
Filed:
Apr. 27, 2023
Jiangsu Academy of Agricultural Sciences, Nanjing, CN;
Baochao Fan, Nanjing, CN;
Bin Li, Nanjing, CN;
Shiying Song, Nanjing, CN;
Xuehan Zhang, Nanjing, CN;
Xuejiao Zhu, Nanjing, CN;
Jinzhu Zhou, Nanjing, CN;
Yongxiang Zhao, Nanjing, CN;
Jizong Li, Nanjing, CN;
Rongli Guo, Nanjing, CN;
Weilu Guo, Nanjing, CN;
Xue Zhang, Nanjing, CN;
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES, Nanjing, CN;
Abstract
Disclosed are a method for preparing a porcine-derived interferon-delta 5 (pIFN-δ5) and an application of the pIFN-δ5, where the method for preparing pIFN-δ5 includes the following steps: step S1, obtaining a DNA fragment containing pIFN-δ5 gene through reverse transcription-polymerase chain reaction (RT-PCR) amplification by using the total RNA of pretreated porcine small intestinal epithelial cells IPEC-J2; step S2, inserting the DNA fragment containing pIFN-δ5 gene into an exogenous expression vector to construct a recombinant expression vector for expressing the pIFN-δ5 gene; and step S3, introducing the recombinant expression vector into a suitable host cell, and driving the host cell to express the pIFN-δ5 gene to obtain the pIFN-δ5. The recombinant pIFN-δ5 protein is used to prepare drugs or preparations for inhibiting infection of porcine epidemic diarrhea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), porcine delta coronavirus (PDCoV) and porcine rotavirus (PRoV).