The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 16, 2024

Filed:

Nov. 12, 2021
Applicant:

Life Technologies Corporation, Carlsbad, CA (US);

Inventors:

Chiu Tai Andrew Wong, Orange, CT (US);

Kylan Szeto, East Lyme, CT (US);

Shanti Shankar, Cheshire, CT (US);

Mark Beauchemin, S. Glastonbury, CT (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C12Q 1/6825 (2018.01); C12Q 1/6874 (2018.01); G01N 35/10 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1065 (2013.01); C12Q 1/6825 (2013.01); C12Q 1/6874 (2013.01); G01N 35/1081 (2013.01);
Abstract

A method for sequencing a target polynucleotide includes detecting a first series of nucleotide incorporations complementary to at least a portion of the target polynucleotide. The first series of nucleotide incorporations forms a first complementary polynucleotide. The target nucleotide is secured to a substrate disposed in a sequencing zone of an assembly. The method further includes moving the substrate to which the target nucleotide is secured to a templating zone of the assembly; removing the first complementary polynucleotide when the substrate is disposed at the templating zone of the assembly, the target polynucleotide remaining secured to the substrate; following the removing, moving the substrate to which the target polynucleotide is secured to the sequencing zone; and detecting a second series of nucleotide incorporations complementary to at least a portion of the target polynucleotide, the second series of nucleotide incorporations forming a second complementary polynucleotide.


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