The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 16, 2024

Filed:

Dec. 19, 2018
Applicant:

Miltenyi Biotec B.v. & Co. KG, Bergisch Gladbach, DE;

Inventors:

Rafijul Bari, Gaithersburg, MD (US);

Markus Granzin, Germering, DE;

Wing Leung, Boston, MA (US);

Nina Moker, Cologne, DE;

Volker Huppert, Kurten, DE;

Assignee:

MILTENYI BIOTEC B.V. & CO. KG, Bergisch Gladbach, DE;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
A61K 35/17 (2015.01); C07K 14/54 (2006.01); C07K 14/545 (2006.01); C07K 14/55 (2006.01); C12N 15/867 (2006.01);
U.S. Cl.
CPC ...
A61K 35/17 (2013.01); C07K 14/5443 (2013.01); C07K 14/545 (2013.01); C07K 14/55 (2013.01); C12N 15/867 (2013.01); C07K 2319/85 (2013.01); C12N 2740/13044 (2013.01);
Abstract

The present invention discloses an in-vitro method for transferring biological material into activated NK cells with a pseudotyped retroviral vector particle or a virus-like particle thereof, comprising the steps a) activation of NK cells, and b) addition of said pseudotyped retroviral vector particle or virus-like particle thereof to said activated NK cells, wherein said pseudotyped retroviral vector particle or virus-like particle thereof comprises a modified baboon endogenous retrovirus (BaEV) envelope glycoprotein that is able of binding to and fusing with a hematopoietic cell membrane, thereby transferring biological material into said activated NK cells. Preferentially, the activating of NK cells is performed by the addition of a IL-1 family cytokine to the NK cells.


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