The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 09, 2024
Filed:
Jan. 06, 2019
Applicant:
Nucleix Ltd., Rehovot, IL;
Inventors:
Assignee:
NUCLEIX LTD., Rehovot, IL;
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12N 5/09 (2010.01); C12N 15/10 (2006.01); C12N 15/66 (2006.01); C12Q 1/6827 (2018.01); C12Q 1/6886 (2018.01); G01N 33/574 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6886 (2013.01); C12N 5/0693 (2013.01); C12N 15/1089 (2013.01); C12N 15/66 (2013.01); C12Q 1/6827 (2013.01); G01N 33/574 (2013.01); C12Q 2537/165 (2013.01); C12Q 2565/40 (2013.01);
Abstract
Methods and kits for detection of cancer-related mutations in a DNA sample using enzymatic restriction and real-time PCR. A DNA sample is subjected to digestion with a restriction endonuclease to obtain restriction endonuclease-treated DNA, followed by co-amplification of a restriction locus comprising a cancer mutation site and a control locus. A ratio of signal intensities of the amplification products of the restriction locus and the control locus is used to detect the cancer-related mutation.