The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 09, 2024
Filed:
May. 29, 2020
Applicant:
Hoffmann-la Roche Inc., Little Falls, NJ (US);
Inventors:
Sonja Offner, Penzberg, DE;
Friederike Jung, Penzberg, DE;
Assignee:
Hoffmann-La Roche Inc., Little Falls, NJ (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/0781 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0635 (2013.01); C12N 2500/30 (2013.01); C12N 2501/2301 (2013.01); C12N 2501/2302 (2013.01); C12N 2501/2304 (2013.01); C12N 2501/2306 (2013.01); C12N 2501/231 (2013.01); C12N 2501/2321 (2013.01); C12N 2501/25 (2013.01); C12N 2502/1107 (2013.01); C12N 2529/00 (2013.01);
Abstract
Herein is reported a method for the co-cultivation of single deposited B-cells, which can be of any source, with EL-4 B5 feeder cells in a suitable co-cultivation medium. In the herein reported methods the EL-4 B5 cells have been irradiated with a dose of less than 40 Gy, preferably 9.5 Gy or less. Thereby the EL-4 B5 cells have a higher viability and maintain the ability to divide in cultivation at doses less than 6 Gy.