The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 09, 2024

Filed:

Jun. 29, 2022
Applicant:

Illumina, Inc., San Diego, CA (US);

Inventors:

Sz-Chin Lin, San Diego, CA (US);

Jay Taylor, San Diego, CA (US);

Minsoung Rhee, San Diego, CA (US);

Jennifer Foley, San Diego, CA (US);

Wesley Cox-Muranami, San Diego, CA (US);

Cyril Delattre, San Diego, CA (US);

Tarun Khurana, San Diego, CA (US);

Paul Crivelli, San Diego, CA (US);

Assignee:

Illumina, Inc., San Diego, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); G01N 21/05 (2006.01);
U.S. Cl.
CPC ...
B01L 3/502715 (2013.01); B01L 3/502707 (2013.01); B01L 3/502738 (2013.01); G01N 21/05 (2013.01); B01L 2300/0861 (2013.01); B01L 2400/0622 (2013.01); B01L 2400/08 (2013.01);
Abstract

An example method includes connecting a flow cell to an instrument. The flow cell includes a flow channel including a manifold section having a manifold section swept volume and a detection section having a detection section swept volume. A ratio of the detection section swept volume to manifold section swept volume is at least 10 to 1. A first reagent is pumped through the flow channel. A first chemical reaction is performed between the first reagent and analytes positioned in the detection section. A subsequent reagent is pumped through the flow channel to flush out the remaining reagent. A concentration of at least 99.95 percent of reagent positioned in the detection section is the subsequent reagent, after pumping a total volume of the subsequent reagent through the flow channel that is equal to or less than 2.5 times a total swept volume of the manifold section plus the detection section.


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