The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 02, 2024

Filed:

Aug. 09, 2021
Applicant:

Sharp Life Science (Eu) Limited, Oxford, GB;

Inventors:

Laura Huang, Oxford, GB;

Benjamin James Hadwen, Oxford, GB;

Adam Christopher Wilson, Oxford, GB;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); G01N 15/1031 (2024.01); G01N 15/14 (2006.01); G01N 15/10 (2006.01);
U.S. Cl.
CPC ...
B01L 3/502792 (2013.01); B01L 3/502746 (2013.01); G01N 15/1031 (2013.01); G01N 15/1484 (2013.01); B01L 2200/06 (2013.01); B01L 2200/061 (2013.01); B01L 2400/0427 (2013.01); B01L 2400/084 (2013.01); G01N 2015/1043 (2013.01); G01N 2015/1481 (2013.01); G01N 2015/1486 (2013.01);
Abstract

A microfluidic device performs a method of partitioning droplets from a fluid reservoir containing particles that provides a non-Poissonian distribution of dispensed droplets containing a desired number of particles. Using an electrowetting on dielectric (EWOD) device, droplets are dispensed having a Poissonian distribution of dispensed droplets containing a desired number of particles, and the droplets are interrogated to determine whether each dispensed droplet has a desired number of particles. Droplets that contain the desired number of particles are moved by EWOD operation to a reaction area on the EWOD device, and droplets that do not contain the desired number of particles are rejected and moved by EWOD operation to a holding area on the EWOD device that is different and spaced apart from the reaction area. The result is that droplets in the reaction area have a non-Poissonian distribution of droplets containing the desired number of particles.


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