The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 02, 2024

Filed:

Apr. 22, 2023
Applicant:

Shanghai Sixth People's Hospital, Shanghai, CN;

Inventors:

Yun Qian, Shanghai, CN;

Cunyi Fan, Shanghai, CN;

Xiangyun Yao, Shanghai, CN;

Zhiwen Yan, Shanghai, CN;

Lingchi Kong, Shanghai, CN;

Xu Wang, Shanghai, CN;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
A61L 27/38 (2006.01); A61L 27/44 (2006.01);
U.S. Cl.
CPC ...
A61L 27/3878 (2013.01); A61L 27/383 (2013.01); A61L 27/443 (2013.01); A61L 2430/32 (2013.01);
Abstract

A nerve conduit loaded with adipose-derived stem cells and a preparation method thereof are provided. The preparation method includes: S1, adding polycaprolactone and polyvinylpyrrolidone into a binary organic solvent, performing ultrasonic treatment, and then adding reduced graphene oxide nanoparticles to obtain a spinning solution; S2, electrospinning with the spinning solution and then washing for several times to obtain a semi-finished conduit product; and S3, injecting a cell mixture into the semi-finished conduit product to obtain the nerve conduit. A fiber surface of the nerve conduit has groove structures, and thus a specific surface area and cell adhesion sites are increased, and adhesion and proliferation of cells are facilitated. By loading the adipose-derived stem cells, neurotrophic phenotypic effect of peripheral nerve scaffold is improved, and can effectively avoid immunological rejection of transplantation, promote orientational growth of axons into the nerve conduit and promote myelination effect of Schwann cells.


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