The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 26, 2024

Filed:

Jun. 21, 2023
Applicant:

Hubei University, Wuhan, CN;

Inventors:

Chun-Chi Chen, Wuhan, CN;

Jian-Wen Huang, Wuhan, CN;

Jian Min, Wuhan, CN;

Xian Li, Wuhan, CN;

Beilei Shi, Wuhan, CN;

Panpan Shen, Wuhan, CN;

Yu Yang, Wuhan, CN;

Yumei Hu, Wuhan, CN;

Longhai Dai, Wuhan, CN;

Lilan Zhang, Wuhan, CN;

Yunyun Yang, Wuhan, CN;

Rey-Ting Guo, Wuhan, CN;

Assignee:

HUBEI UNIVERSITY, Wuhan, CN;

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 9/18 (2006.01);
U.S. Cl.
CPC ...
C12N 9/18 (2013.01); C12Y 301/01074 (2013.01);
Abstract

A modified cutinase is disclosed. The cutinase has the modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of asparagine at position 181 with alanine, or substitutions of asparagine at position 181 with alanine and phenylalanine at position 235 with leucine. The modified enzyme has improved PET-hydrolytic activity, and thus, the high-activity PET hydrolase is obtained, and the industrial application value of the PET hydrolase is enhanced.


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