The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 26, 2024

Filed:

Jun. 12, 2017
Applicant:

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

Xiaoge Guo, Brookline, MA (US);

Alejandro Chavez, New York, NY (US);

Max Schubert, Brookline, MA (US);

Eric Kelsic, Boston, MA (US);

Assignees:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C12N 15/00 (2006.01); C12N 15/86 (2006.01); C12N 15/90 (2006.01); C40B 40/08 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1037 (2013.01); C12N 15/00 (2013.01); C12N 15/102 (2013.01); C12N 15/1058 (2013.01); C12N 15/86 (2013.01); C12N 15/905 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01); C40B 40/08 (2013.01);
Abstract

Expressing guide nucleic acids (e.g., gRNA) from the same oligonucleotide that contains donor sequence permits the high efficiency, simultaneous transformation of a population of cells with both substrates. Using oligonucleotide chip array technology, one can construct thousands of oligonucleotides with customized gRNA and donor sequence in a cost effective manner. In combination, one can efficiently modify endogenous and exogenous genes.


Find Patent Forward Citations

Loading…