The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 12, 2024

Filed:

May. 21, 2020
Applicant:

Fuwai Hospital Chinese Academy of Medical Sciences, Shenzhen, Shenzhen, CN;

Inventors:

Shengshou Hu, Beijing, CN;

Guangmao Liu, Beijing, CN;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
A61F 2/24 (2006.01); A61F 2/82 (2013.01); A61M 60/117 (2021.01); G01N 15/06 (2006.01); G01N 33/49 (2006.01);
U.S. Cl.
CPC ...
A61F 2/2472 (2013.01); A61F 2/82 (2013.01); A61M 60/117 (2021.01); G01N 15/0606 (2013.01); G01N 2015/018 (2024.01); G01N 15/075 (2024.01); G01N 33/4905 (2013.01);
Abstract

A method for in vitro simulation and evaluation of platelet adhesion in blood-contacting medical devices is disclosed, including the following steps: (1) using a glycerin aqueous solution with a mass percentage concentration of 40% in an extracorporeal circulation circuit to simulate a viscosity and hydrodynamic characteristics of blood, and adding fluorescent particles with a diameter of 3 μm to 5 μm to the solution to simulate platelets; (2) after the solution circulates in the circuit for a specified time period, removing flow passage components of a tested device, and observing the deposition of the fluorescent particles on a blood-contacting surface inside the device by naked eyes and photographs; and (3) using laser-induced fluorescence (LIF) technique to apply laser light on a device surface deposited with the fluorescent particles and in contact with blood, and using charge-coupled device (CCD) camera imaging to photograph the aggregation and adhesion of laser-induced fluorescent particles.


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