The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 05, 2024
Filed:
Oct. 19, 2019
Applicants:
Shaofeng Ding, Santa Fe Springs, CA (US);
Qiang Liu, Rancho Cucamonga, CA (US);
Inventors:
Shaofeng Ding, Santa Fe Springs, CA (US);
Qiang Liu, Rancho Cucamonga, CA (US);
Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6844 (2018.01); G01N 21/76 (2006.01);
U.S. Cl.
CPC ...
G01N 21/76 (2013.01); C12Q 1/6844 (2013.01);
Abstract
A new fluorescence detection method called pyrophosphorolysis activated fluorescence was developed to measure PAP amplification of nucleic acid. A fluorophore-quencher dual-attached blocked primer was used for PAP which has a fluorophore attached to a nucleotide in the internal region or at the 5′ end and a quencher attached to a blocked nucleotide at the 3′ end. Multiple fluorophore-quencher dual-labeled blocked primers were also used for multiplex PAP, which are attached with different fluorophores to distinguish multiple templates in a reaction.