The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 27, 2024

Filed:

Sep. 02, 2022
Applicant:

The Trustees of the University of Pennsylvania, Philadelphia, PA (US);

Inventors:

Mark I. Greene, Penn Valley, PA (US);

Douglas B. Cines, Wynnewood, PA (US);

Zheng Cai, Wynnewood, PA (US);

Zhiqiang Zhu, Philadelphia, PA (US);

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 33/577 (2006.01); G01N 33/53 (2006.01); C07K 16/18 (2006.01); G01N 33/68 (2006.01); C07K 16/24 (2006.01); G01N 33/86 (2006.01); A61K 39/395 (2006.01); A61K 38/19 (2006.01); C07K 14/52 (2006.01); A61K 39/00 (2006.01);
U.S. Cl.
CPC ...
G01N 33/6863 (2013.01); A61K 38/195 (2013.01); A61K 39/3955 (2013.01); C07K 14/522 (2013.01); C07K 16/24 (2013.01); G01N 33/86 (2013.01); A61K 2039/505 (2013.01); G01N 2333/522 (2013.01); G01N 2400/40 (2013.01); G01N 2800/222 (2013.01); G01N 2800/50 (2013.01); G01N 2800/52 (2013.01);
Abstract

The present invention provides a humanized antibody or antibody fragment comprising (a) a humanized light chain comprising 1) Complementarity Determining Region (CDR)-L1, the sequence of which is identical to the sequence of SEQ ID NO: 3; 2) CDR-L2, the sequence of which is identical to the sequence of SEQ ID NO: 4; and 3) CDR-L3, the sequence of which is identical to the sequence of SEQ ID NO: 5, and (b) a humanized heavy chain comprising 1) CDR-H1, the sequence of which is identical to the sequence of SEQ ID NO: 6; 2) CDR-H2, the sequence of which is identical to the sequence of SEQ ID NO: 7; and 3) CDR-H3, the sequence of which is identical to the sequence of SEQ ID NO: 8, as well as methods for treating, diagnosing, and monitoring the progression of HIT. The present invention also provides methods for assessing the antigenicity and ability to cause HIT of anionic anticoagulants. The present invention also provides a mutant protein which has the same amino acid sequence of a wild type PF4 monomer except that (i) at least one amino acid of the wild type PF4 monomer has been deleted, (ii) at least one amino acid of the wild type PF4 monomer has been replaced by another amino acid, or (iii) a combination of such changes has been made. The present invention also provides methods of treating or reducing the likelihood of HIT, treating angiogenesis, treating abnormal cell growth, or affecting coagulation pathologies that lead to thrombus formation, by administering such mutant proteins to a patient.


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